Byron W. Purse
Associate Professor, Organic Chemistry
office: CSL 213
- Assistant Professor, San Diego State University, 2013.
- Assistant Professor, University of Denver, 2008.2013
- Postdoctoral Research Associate, Bäckvall Group, Stockholm University, Sweden, 2006.2008
- PhD in Organic Chemistry, Rebek Group, The Scripps Research Institute, La Jolla, California, 2000.2005
- BSc High Honours in Chemistry and Biochemistry, University of Regina, Canada, 1996.2000.
In the Purse Lab, we are interested in using molecular design and synthetic organic chemistry to create new molecules that can serve in practical applications and be used to study the fundamental relationships between molecular structure and properties. With new molecules in hand, we use the tools of modern physical organic chemistry to study their properties, including self-assembly, molecular encapsulation, fluorescence, and the ability of some of our molecules to functional as molecular probes or prospective medicines. Students working in the Purse Lab can expect to gain hands-on experience in current synthetic methodology, organic compound characterization, methods for studying the kinetics and thermodynamics of reactions and molecular complex formation (NMR, ITC, optical spectroscopy), and recently biochemistry! We are always interested in new co-workers with a passion for knowledge, a drive for success, a creative mindset, and a dedication to the pursuit of excellence.
Nucleosides are the building blocks of DNA and RNA, the molecules that store the genetic code. In addition to this role, they are used as the energy currency of cells (ATP is a nucleoside triphosphate), for controlling chemistry in cells (catalytic RNA in ribosomes), and as chemical transporters (tRNA). Their importance cannot be understated! Chemists have an interest in modifying the structures of nucleosides to make new molecular probes (e.g. fluorescent molecules that can be used to track and study nucleic acids) and to make drugs. Many important medicines are synthetic derivatives of nucleosides that “trick” infected or malignant cells into using the “unnatural” nucleoside by mistake during replication. This mechanism is how azidothymidine (AZT) attacks the HIV virus, gemcitabine kills breast cancer cells, and acyclovir slows the spread and growth of the Herpes virus. Back to the former application, fluorescent nucleoside analogues are used to study how enzymes in our body work and for modern, next-generation DNA sequencing methods.
Our lab is interested in developing new nucleoside analogues both for unmet needs as molecular probes and in medicinal chemistry. We are currently working on synthetic elaboration of natural nucleosides to make the nucleobases fluorescent, while minimizing the biochemical impact of the required structural changes. One of the applications of these compounds is to create a fluorescent version of DNA using the PCR reaction! New compounds designed and prepared by our lab have promising photophysical properties, and we are currently testing their applications as molecular probes. Relatedly, we are examining chemical methods for delivering analogues of nucleoside triphosphates across the plasma membranes of living cells. These methods are designed for delivering both molecular probes and drugs. As such, we are contributing new tools for understanding biology and testing new approaches to treat disease.
The field of supramolecular chemistry is concerned with the study of how and why molecules can self-assemble into higher-order structures with emergent properties. Throughout the history of the field, most studies and applications have used assemblies at equilibrium, where there is dynamic exchange of components between assemblies. Our group is interested in self-assembled supramolecules with high kinetic stability; that is, those whose components exchange slowly or not at all. Such assemblies can be useful in environments where component exchange cannot be tolerated, such as open systems with flow.
Recently, we have identified the most kinetically stable known molecular capsules that are held together by hydrogen bonds. These capsules will indefinitely trap their occupants at ambient temperatures even as thermodynamics would prefer the release of the capsules' guests. Heat is required to break the capsules apart and release the small guests. Noting the special stability of these capsules, we have turned our attention towards embedding these capsules in polymers so as to harness mechanical forces to control occupancy. The long-term goal of our work in this area is to create new smart materials that can release or take up specific small molecules in response to mechanical forces, for example, to modulate the properties of polymers or to indicate and measure polymer stress that might lead to material failure. We are also investigating the use of our capsules to control chemicals reactions by compartmentalization, similarly to how cells separate chemical environments using their organelles.
- Burns, Dillon D.; Teppang, Kristine L.; Lee, Raymond W.; Lokensgard, Melissa Eleanor; Purse, Byron W. Fluorescence turn-on sensing of DNA duplex formation by a tricyclic cytidine analogue. J. Am. Chem. Soc. 2017, (in press). doi: 10.1021/jacs.6b10410.
- Rodgers, B.J.; Elsharif, N.A.; Vashisht, N.; Mingus, M.M.; Mulvahill, M.A.; Stengel, G.; Kuchta, R.D.; Purse, B.W. Functionalized Tricyclic Cytosine Analogues Provide Nucleoside Fluorophores with Improved Photophysical Properties and a Range of Solvent Sensitivities. Chem. Eur. J. 2014, 20, 2010–2015. doi:10.1002/chem.201303410.
- Chapin, J.C.; Purse, B.W. Guest loading, chromatographic purification, and controlled release from kinetically trapped, hydrogen-bonded pyrogallolarene capsules. Supramol. Chem. 2014, 7–8, 517–520. doi:10.1080/10610278.2013.863314.
- Mehdizadeh, E.; Chapin, J.C.; Gonzales, J.M.; Rahafrooz, A.; Abdolvand, R.; Purse, B.W.; Pourkamali, S. Microelectromechanical disk resonators for direct detection of liquid-phase analytes. Sensor Actuat A–Phys 2014, 216, 136–141. doi:10.1016/j.sna.2014.05.022.
- Chapin, J.C.; Kvasnica, M.; Purse, B.W. Molecular Encapsulation in Pyrogallolarene Hexamers Under Nonequilibrium Conditions, J. Am. Chem. Soc. 2012, 134, 15000–15009. doi:10.1021/ja305252t.
- Stengel, G.; Purse, B.W.; Kuchta, R.D. Effect of transition metal ions on the fluorescence and Taq-catalyzed polymerase chain reaction of tricyclic cytidine anologues, Anal. Biochem. 2011, 416, 53–60. doi:10.1016/j.ab.2011.04.033.
- Kvasnica, M.; Chapin, J.C.; Purse, B.W. Efficient Loading and Kinetic Trapping of Hexameric Pyrogallolarene Capsules in Solution, Angew. Chem. Int. Ed. 2011, 50, 2244–2248. doi:10.1002/anie.201007800.
- Stengel, G.; Urban, M.; Purse, B.W.; Hocek, M.; Kuchta, R.D. Incorporation of the fluorescent nucleotide analogue tCTP by T7 RNA polymerase, Anal. Chem. 2010, 82, 1082–1089. doi:10.1021/ac902456n.
- Stengel, G.; Urban, M.; Purse, B.W.; Kuchta, R.D. High density labeling of PCR products with the fluorescent analogue tCo, Anal. Chem. 2009, 81, 9079–9085. doi:10.1021/ac9017555.